Fig. 5. Atypical localization of KLP61F in spermatocytes. Wild-type testes stained with antibodies against KLP61F (red), a fluorescent chromatin dye (blue) and -tubulin (green, A-C) and -tubulin (lavender, D). (A-D) Single projection of a stack of optical sections. Spermatocytes at the G2/M transition failed to show KLP61F enrichment at centrosomes (A) or spindle localization at metaphase (B). Spermatocytes are shown at telophase of meiosis II (C) and meiosis I (D) with septin-like localization of KLP61F. (E) Low magnification wide-field image of a cyst. Asterisks indicate spermatocytes at higher magnification in metaphase (F), anaphase (G) and late anaphase/telophase (H). Bar (A-D), 2 µm; (E), 10 µm; (F-H), 5 µm.