Fig. 5. In vivo time-lapse analysis of growth of adult stages of wild-type hyphae. Mycelium from exponentially grown liquid YPD cultures was transferred to slides and growth was monitored. Images represent frames from Movie 3 (see supplementary material) at the corresponding time points (hours:minutes). Septal sites are marked by asterisks. Cytoplasmic flow as visible by the movement of large vacuoles is indicated by arrows. Arrowheads mark the same vacuole at different time points. Septation alters the direction of flow and flow was also reversed in one hypha. Scale bar, 40 µm.