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Fig. 4. Visualization of the subcellular distribution of PKC-ßII and PKC-{gamma} by electron microscope immunocytochemistry in oocytes 30 and 90 minutes after meiosis reinitiation. Oocytes treated with anti-PKC-ßII antibody 0-30 minutes (A) or 60-90 minutes (B,C) after release from the follicle. Oocytes treated with anti-PKC-{gamma} antibody 0-30 minutes (D) or 60-90 minutes (E) after release from the follicle. For each cPKC isoform, changes in the distribution of gold particles in the cytoplasm (gray columns) and in the nucleus (black columns) over time are shown in the two histograms: for PKC-ßII, the number of gold particles increased in the nucleus [in the nucleoplasm (Np) as well as in the interchromatin granules (IG)] and in the cytoplasm (Cy); for PKC-{gamma}, the nuclear envelope (NE, white arrow) and the nucleus were free of gold particles but the number of gold particles, although smaller than for other isoforms, increased over time. Black arrows indicate the gold particles on the NE. Magnification 20,000x. *, statistically significant difference between cytoplasm and nucleus, P<0.05.