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Fig. 6. Heat shock affects mobility of GFP-Hsp70 in the nucleus and GFP-Hsp70(K71E) in the nucleus and cytoplasm. HeLa cells were heat shocked and the fluorescence recovery after photobleaching was measured. (A) fluorescence recovery of GFP vector in the nucleus({circ}), GFP-Hsp70 in the cytoplasm ({triangleup}), GFP-Hsp70 in the nucleoplasm ({blacktriangleup}) and GFP-Hsp70 in the nucleolus (solid circles). (B) fluorescence recovery of GFP-Hsp70(K71E) in the cytoplasm () and nucleoplasm ({blacktriangleup}).