Fig. 4. EGF-induced re-distribution of Eps15 depends on Y1045 of the EGFR. CHO cells co-expressing FLAG-tagged Eps15, c-Cbl and wild-type EGFR (A-F) or EGFR-Y1045F (G-L) were incubated with EGF-TxR (red) on ice for 1 hour. After stimulation at 37°C for 0 minutes (A,B,G,H), 1 minute (C,D,I,J) or 5 minutes (E,F,K,L), cells were fixed and stained with anti-FLAG, followed by FITC-conjugated secondary antibody (green). Cells were analyzed by confocal microscopy. The displayed confocal planes are from the basal half of the cells. XZ sections are shown below each panel.