Fig. 3. Colocalization of FIP with filamin in aggregating AX2 cells as analyzed by confocal microscopy. Cells were starved for 10 hours in shaking suspension, fixed with methanol and stained with FIP-specific mAb K12-362-8 (A) or K12-454-2 (D) and a polyclonal filamin serum (B,E). C and F show the overlays. Detection was with Alexa488-conjugated goat anti-mouse IgG and Alexa568-labeled goat anti-rabbit IgG. The arrows point to areas of colocalization. Bar, 10 µm.