Fig. 7. Cytoskeletal association of the GFP-cFIP fusion protein during cytoskeletal rearrangements in response to cytochalasin A. Dictyostelium AX2 cells expressing the GFP-cFIP fusion protein were attached to coverslips and treated with 20 µM cytochalasin A in phosphate buffer for 1 hour prior to fixation with methanol. In the upper panel, a cell labeled with the filamin-specific mAb 82-382-8 and, in the lower panel, a cell stained with the actin-specific mAb Act 1-7 is shown (A,B, GFP fluorescence; A',B', antibody staining; A",B", overlay). The merged pictures (A",B") reveal a cytoskeletal association of the GFP-cFIP fusion protein. It colocalizes with the cytoskeletal elements in patches at the plasma membrane. Bar, 5 µm.