JCS -- van der Laan et al. 117 (21): 5023 Figure IG8

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Fig. 8. Immunoexpression of RDB proteins downstream of HR6a/b-Rad18^Sc is low in XY body chromatin of primary spermatocytes. (A) Left: Immunostaining of UBC13 using ${alpha}$ -UBC13 (green) shows that UBC13 is absent from a subnuclear region where the XY body is situated (arrow). Right: Co-immunostaining of UBC13 and Sycp3 (red) to visualize the autosomes and the location of the XY body. (B) Left: Immunostaining of the TLS polymerase pol ${eta}$ by anti-pol ${eta}$ (green). The level of this RDB protein is also very low in the subnuclear region where the sex chromosomes are located (arrow). Right: Co-immunostaining of pol ${eta}$ and Sycp3 (red) to visualize the autosomes and the location of the XY body. Bar, 20 µm.