Fig. 1. Dronc is processed at E352 when produced in E. coli. (A) Full-length Dronc protein. Mutations to alanine were made at the indicated residues. (B) Coomassie-blue stain of recombinant proteins separated by SDS-PAGE. Wild-type Dronc and the D113A and D135A mutants were fully processed, whereas E352A Dronc remained mostly full length. The migration of mass markers is indicated to the left of the gel in kDa.