Fig. 5. Drice is required for apoptosis in S2 cells. (A) Cells were either mock treated or treated with cat dsRNA or drice dsRNA for 20 hours before the addition of diap1 dsRNA. Cell viability was determined at 6 hours, 12 hours and 24 hours after the addition of diap1 dsRNA. (B) An anti-DIAP1 immunoblot of S2 cells pretreated with drice dsRNA and then diap1 dsRNA, and harvested at the times shown after diapl dsRNA addition. (C) An anti-Dronc immunoblot of S2 cells pretreated with drice dsRNA and then diap1 dsRNA, and harvested at the times shown after diapl dsRNA addition. The double asterisk represents a non-specific band detected by the Dronc antiserum.