Fig. 10. Localization of endogenous SmAV, F-actin, nonmuscle myosin II, CaP and ERK1/2 before and after PDBu treatment of A7r5 smooth-muscle cells. (A) In untreated A7r5 cells, endogenous SmAV (a) appears to be membranous, whereas F-actin (b,e,h), nonmuscle myosin II (d) and CaP (g,k) are primarily associated with microfilaments, and ERK1/2 appears to be cytosolic (j). (B) After PKC activation with PDBu, endogenous SmAV (a,g), F-actin (b,e), nonmuscle myosin II (d), CaP (h,k) and ERK1/2 (j) all localize to podosome-like structures (arrowheads). SmAV, F-actin, myosin IIB and CaP also concentrate at or near membrane ruffles (arrows). Merged images (c,f,i,l) show the overlap of signal from panels on the left (green) with the signal from the middle panels (red); the overlap appears yellow. Scale bar, 25 µm.