Fig. 4. CaP and the supervillin C-terminus colocalize in COS-7 cells. Confocal images of immunostained transfected COS-7 cells. (A, left) Single expression of the EGFP-tagged C-terminal 783 residues of bovine supervillin (EGFP-c-SV). (A, right) Single expression of h1-CaP. (B) CaP (left) and EGFP-c-SV (middle) co-expressed in COS-7 cells. Merged image is shown to the right; regions of signal overlap appear in yellow. (C) Co-expression of CaP and EGFP control. CaP was visualized by staining with an anti-CaP antibody and EGFP-c-SV was visualized with an anti-GFP antibody. Scale bar, 10 µm.