Fig. 2. ST6GalNAc I overexpression alters cell shape. (A) Phase contrast microphotographs of STn-control cells and of STn+ ST6GalNAc I transfectants illustrating morphological differences between the two cell types. (B,C) Quantitative analysis of morphological parameters was performed on three STn-control clones and three STn+ clones. Cell area and perimeter were acquired for each cell type and the shape factor (f) was calculated as described in Materials and Methods. Error bars represent the standard deviation for 100 cells. (D) Rhodamine-labeled phalloidin was used to detect polymerized actin on fixed and permeabilized cells cultivated on glass lamellae. (E) Focal contacts were visualized using an anti-vinculin mAb. Pictures from representative fields are shown.