Fig. 5. STn epitopes are carried by various glycoproteins, among which is the integrin ß1 subunit. (A) Total Triton X-100 cell extracts of the STn-clones (B10 and F4) and of the STn+ clones (C2 and G9) were submitted to SDS-PAGE in nonreducing conditions and to western blotting. Blots were probed with the anti-STn mAbs TKH2 and B72.3 and the anti-ß1 mAb KMI6. (B) Total cell extracts were immunoprecipitated with the anti-STn TKH2, and immunoprecipitates were submitted to western blotting using either the anti-ß1 KMI6 or the anti-STn TKH2. The bands marked by a star correspond to the immunoprecipitating immunoglobulin.