Fig. 5. Polarised distribution of the GPI-anchored MDP constructs in CaCo-2 cells. (A,B) CaCo-2 cells stably expressing either wtMDP (A) or MDPgly (B) were seeded on polycarbonate filters at confluent levels and grown for 14 days to allow them to polarise with feeding every other day. (i) Anti-MDP was visualised with Alexa488 (green) and (ii) anti-Na⁺/K⁺ ATPase antibody with Alexa594-conjugated secondary antibody (red). (iii) Merged images with colocalisation of the two antibodies appearing yellow. (iv) Z-axis images of cells in (iii). Bar, 10 µm. The images are representative of three independent experiments.