Fig. 6. myr-HA-ß₂AR-C is phosphorylated by an unidentified kinase(s) in GVBD oocytes. Xenopus oocytes injected with the indicated mRNAs were incubated for 24 hours before the addition of progesterone. Following progesterone-induced GVBD, oocytes were lysed and analyzed by 2D electrophoresis and immunoblotting with hemagglutinin (HA). Representative examples from two independent experiments are shown. X, multiple phosphorylated forms of myr-HA-ß₂ARC.