Fig. 6. Cortactin expression is necessary for actin-dependent N-cadherin adhesion strengthening. (A) Cortactin gene-silenced samples were incubated with recombinant N-cad-Fc-coated beads and jet washed in a logarithmic series following a 15-minute incubation period. Cells within a sample were divided into two groups based on cortactin expression levels. The number of beads bound per cell was quantified. Representative images for samples in the second and sixteenth washes show the degree of bead binding to cells for high and low levels of cortactin. Bar, 20 µm.