Fig. 4. Loss of fluorescence from YP::GFP reporter gene and accumulation of YP170 in Caenorhabditis elegans cpl-1 mutant embryos. YP170::GFP fluorescence in oocytes and embryos in the gonad of wild-type (A) and cpl-1 mutant (C) hermaphrodites. B and D are Nomarski images of A and C, respectively. The vulva is indicated by an arrow. YP170::GFP in wild-type embryo at approximately 28-cell stage (E) and threefold stage (F). Significantly decreased levels of fluorescence are observed in similarly aged cpl-1(ok360) embryos (H,I). Immunofluorescence with antibody to YP170 in wild-type (G) and cpl-1 mutant (J) embryos. Each embryo is approximately 50 µm in length.