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Fig. 5. Immuno electron microscopy (IEM) on wild-type and cpl-1(ok360) adult Caenorhabditis elegans hermaphrodites with YP170 antibody. (A) Antibody localises to small yolk platelets in wild-type embryos. (B) Higher magnification of wild-type embryo. (C) Mutant (ok360) embryo showing accumulation of enlarged yolk platelets. Smaller yolk vesicles can also be observed in some embryonic cells. (D) Higher magnification of enlarged yolk vesicles in cpl-1(ok360) embryo. (E,F) Double labelling of yolk platelets with anti-pro-CPL-1 antibody (detected with 10 nm gold; arrowheads) and anti-YP170 antibody (detected with 15 nm gold; arrows) in a wild-type embryo (E) and in the hermaphrodite pseudocoelom (F). Bar, 500 nm.