JCS -- Fiumara et al. 117 (21): 5145 Figure IG1

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Fig. 1. Time-course and stoichiometry of apSyn phosphorylation by distinct protein kinases. Purified bovine synapsin I (5 pmol; closed symbols) or recombinant apSyn (clone 11.1; 50 pmol; open symbols) cleaved from GST and repurified was incubated at 30°C for the indicated time periods with either the catalytic subunit of PKA (A), CaMK II (B), MAPK/Erk2 (C) or PKC (D) in the presence of radioactive ATP. Phosphorylation stoichiometries (mol [³²P]/mol synapsin), calculated at various incubation times, are shown as mean±s.e.m. (n=4-5).