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Fig. 7. Detailed analysis of lifetime components in NGF-stimulated cells. COS7 cells expressing TrkA-Ros-ECFP and EYFP-SHP1-SH2 were stimulated with 100 ng/ml NGF for 20 minutes. Cells were fixed and confocal and fluorescence lifetime images were recorded. (A) Left panel: LSM image with superimposed ECFP and EYFP channel. Right panel: the quantitative distribution of ECFP (blue curve) and EYFP (yellow curve) along the line depicted in the left panel is shown. (B) Color-coded images of the amplitude weighted mean lifetime ({tau}m), the fast lifetime component ({tau}f) and its amplitude (Af). The color scale used is shown in the respective panels of C. (C) Distribution of the parameters visualized in B over the entire cell.