Fig. 8. C. elegans sdn-1 (ok449) mutants express a protein recognized by antibodies against the phosphorylated syndecan-4 epitope, with immunoreactivity localizing to the nerve ring. (a) Anti-P-syndecan-4 immunoreactivity in sdn-1 (ok449) mutants. (b,c) Double immunostaining of anti--heparan sulfate and anti-P-syndecan-4 immunoreactivity in sdn-1 (ok449) mutants respectively. (d) Merged images of b and c. The insert shows an enlarged area, with detail of the heparan sulfate proteoglycans (green) and their close localization to the phosphorylated SDN-1 (red). White arrowheads indicate the immunoreaction at the nerve ring. The image in panel a corresponds to a 3D reconstruction of an image stack, whereas the rest represent two optic slices. Bar, 20 µm.