Fig. 2. Full-length Xin is targeted to actin containing structures upon transient transfection. PtK2 cells were transfected with Xin-EGFP. Cells were fixed 48 hours after transfection, and stained with CPITC-phalloidin to visualize F-actin. The Xin-EGFP polypeptide (A,C-E, green) is targeted to F-actin-containing structures (B-E, blue), including cell-cell contacts (C, arrows), focal adhesions (D, arrows) and stress fibres (E, arrows). Scale bar, 10 µm.