Fig. 3. The Xin-repeat region and truncated variants are targeted to actin-containing structures. A7r5 cells were transfected with XR1-16 (A,B), XR1-6 (C,D), XR 4-6 (E,F), XR3-4 (G,H) or XR10-11 (I,J), fixed and stained with CPITC-phalloidin (A-F) or analysed using live imaging to exclude fixation-induced association of EGFP with stress fibres (G-J). For the latter images, localization of the EGFP-tagged construct is shown together with the phase-contrast image. Scale bar, 10 µm.