Fig. 1. CapG-EGFP expression triggers invasion of MDCK-AZ cells. (A-A') Epifluorescence microscopic images of MDCK-C cells and corresponding Texas-red phalloidin (F-actin) staining. (B-B') Distribution of endogenous CapG in MDCK-AZ cells and corresponding F-actin staining; (C) Control staining for CapG in the presence of excess antigen; (C') DAPI; (D) EGFP-expressing MDCK-AZ cells (MDCK-E); (D') F-actin staining. (E) CapG localization in HeLa cells. Bar in A and D, 40 µm; bar in B, C and E, 50 µm. (F) Western blot analysis on cytosolic proteins from MDCK-C clones. CapG-EGFP expression levels relative to endogenous CapG are indicated. (G) Collagen invasion index of seven independent MDCK-C clones (lanes 5-11). MDCK-AZ (lane 1) and MDCK-E cells (lanes 2-3) were used as negative controls; DHD-FIB = positive control (lane 4). cl1 = clone1; clC = clone C. Data are means ± s.e.m. of three independent experiments.