Fig. 5. CapG represses transcriptional activity of VP16 in a dose-dependent manner. HEK 293T cells were transiently transfected with a Gal4-dependent reporter plasmid (p(Gal4)₂50hu.IL-6P-luc+) and pGal4-VP16, either alone or in combination with increasing amounts of pNLS-Gal4-CapG (pGal4-CapG), as indicated. Increasing amounts of NLS-Gal4-DBD-CapG caused a decrease in reporter activity. Luciferase activities were normalized using ß-galactosidase as an internal control. Data are means ± s.e.m. of three independent experiments.