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Fig. 4. Septins and Cdr2p localize independently. (A) spn1-CFP cdr2-YFP (KGY719) cells were grown in YE medium at 25°C and both Spn1p-CFP (red channel) and Cdr2p-YFP (green channel) were photographed and then the images merged. Individual cells are outlined with gray lines. (B) cdr2-GFP spn4{Delta} (KGY718) cells were grown at 25°C and Cdr2p-GFP was visualized in live cells. (C) spn3-GFP cdr2{Delta} (KGY138) cells were grown at 25°C and Spn3p-GFP was visualized in live cells. Cells are also rotated in the Z-axis to show Spn3p ring organization (lower panel). (D) spn3-GFP (KGY3244) cells were transformed with pREP1-cdr2+ and grown in the absence of thiamine to induce expression for 20 hours at 32°C. Images of live cells were captured. Cells are also rotated in the Z-axis to show Spn3p ring organization (lower panel).