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Fig. 7. Structure/function analysis of Cdr2p. A cdr2::ura4+ leu1-32 strain (KGY1519) was transformed with (A) empty pREP1 vector, (B) pREP1cdr2+, (C) pREP1cdr2{Delta}747-775, (D) pREP1cdr2{Delta}730-775, (E) pREP1cdr2{Delta}679-775, (F) pREP1cdr2{Delta}332-775, (G) pREP1cdr2{Delta}730-746 or (H) pREP1cdr2E177A. Transformants were grown to mid-log phase in selective medium. Cells were then collected, filtered, washed, and released into selective medium lacking nitrogen for 48 hours, at which time phase micrographs were taken. Bar, 10 µm.