Fig. 2. NF-YB and NF-YC bind P/CAF and NF-YB also binds p300. COS-1 cells were co-transfected with various Flag-NF-Y subunits and HA-P/CAF, p300, CBP plasmids or HA-vector, as indicated. Cells were lysed and immunoprecipitated with HA antibody and immunoblotted with Flag antibody. Cell lysate was divided and used for immunoprecipitation (IP) or immunoblotting (IB). The transferred membrane was immunoblotted with Flag-M2 antibody (upper panel). The expression of HA-tagged P/CAF, p300 or CBP was confirmed using HA antibody (lower panel).