Fig. 1. Intracellular localisation of thymosin ß₄ in MCF-7 cells detected by immunofluorescence. (A) MCF-7 cells grown to near confluence were stained with the affinity-purified anti-thymosin ß₄ antibody. The cells were counterstained with Oregon Green DNase I (B) in order to label specifically monomeric actin and with the chromatin-specific dye Hoechst 33258 (C). Magnification, x400.