Fig. 4. Treatment of digitonin-permeabilised cells with fluorescently labelled thymosin ß₄. (A-A'') HeLa cells were permeabilised with digitonin and thereafter incubated with fluorescently labelled thymosin ß₄ (A') and the chromatin specific dye chromomycin (A) with the merged image shown in A''. Note the pure cytoplasmic distribution of thymosin ß₄. (B-B'') HeLa cells were permeabilised with digitonin and further treated using 0.2% Triton X-100. (B) chromomycin staining, (B') labelled thymosin ß₄, (B'') merged image. Bar, 50 µm.