Fig. 6. Microinjection of EDC crosslinked thymosin ß₄:actin complex into A431 cells. (A-D) A431 cells were microinjected with crosslinked complex of thymosin ß₄ and ADP-ribosylated actin; note the cytoplasmic distribution of this complex at two hours (A,B) and three hours (C) after microinjection. Microinjected cells are marked with arrows. In C, a microinjected cell is shown whose nucleus is outside the confocal plane (arrow head). (D) A confocal Z-stack image series of a microinjected cell (plane distance 6.4 µm). Bars, 20 µm.