Fig. 3. Analysis of the interaction of 5-HT₄R C-termini with specific sets of proteins by immunoblotting. C-t(a), C-t(b) and C-t(e) peptide baits were incubated with protein extracts from either whole mouse brain (A) or mouse colliculi neurons in primary culture (B). Proteins retained by affinity were separated by SDS-PAGE and transferred electrophoretically onto nitrocellulose sheets. Immunoblotting was performed with antibodies raised against the indicated proteins (anti-Ulip2, 1:2,000; anti-NHERF, 1:500; anti-Veli1-3, 1:200; anti-nNOS, 1:500). For each protein, the immunoreactive signals were found at molecular weights identical to those observed in silver-stained 2D gels. Input represents 5% of the total protein amount used in pull-down experiments. The data illustrated are representative of three experiments.