Fig. 8. Interaction of CIPP with the 5-HT_4(e)R in transiently transfected COS-7 and NIH-3T3 cells. (A) COS-7 cells were transfected with Flag-tagged CIPP (1), the cMyc-5-HT_4(e)R (2), the cMyc-5-HT_4(b)R (3), the cMyc-5-HT_4(e) PVPV R (4) or Flag-tagged CIPP together with each 5-HT₄R construct (5-13) using Lipofectamine^TM 2000. Cells were examined by confocal microscopy. (left) Flag staining (1:1,000); (middle) Myc staining (1:400); (right) merged images. (B) NIH-3T3 cells were transfected with the same constructs using Lipofectamine 2000. Merged images obtained after Flag and Myc staining of cells co-transfected with Flag-tagged CIPP and the cMyc-5-HT_4(e)R (14), the cMyc-5-HT_4(b)R (15) or the cMyc-5-HT_4(e) PVPV R (16) are depicted. Bars, 10 µm.