Fig. 2. Alignment of amino acid sequences of mCx39 and its putative orthologue hCx40.1. Potential cAMP-, PKC- and casein kinase II (CKII)-phosphorylation sites within the cytoplasmic loop and C-terminus are indicated in bold italics and those within extracellular or transmembrane domains were omitted, owing to their inaccessibility to kinases. In mCx39, one cAMP (position 203; R/K-X-X-S/T), three PKC (positions 170, 203 and 300; S/T-X-R/K) and six CKII (positions 190, 236, 290, 296, 310 and 319; S/T-X-X-D/E) phosphorylation sites were found. In hCx40.1, two cAMP (positions 177 and 282), six PKC (pos. 195, 235, 236, 277, 281 and 303) and four CKII (pos. 151, 251, 301 and 325) phosphorylation sites were found. Putative transmembrane regions are boxed. Asterisks, conserved residues; double dots, similar residues; single dots, like residues. Note that both sequences display gaps when aligned by the algorithms of the HUSAR-derived program `sequence pair comparison'. The nucleotide/protein sequences of both connexins have been deposited in the GenBank/EMBL/DDBJ databases under accession numbers AJ414562 (mCx39) and AJ414564 (hCx40.1).