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Fig. 4. RT-PCR analysis of mCx39 expression in different tissues and of different connexins in the diaphragm of the mouse. (A,B) The presence of mCx39 mRNA in various embryonic (ED16.5) and neonatal (P0) tissues of mice is confirmed by using an intron-spanning primer combination to amplify a 1.1 kb product. Amplification of a 243 bp product specific for ß-actin cDNA (De Sousa et al., 1993) demonstrated the quality of the reverse-transcribed cDNA except for neonatal eye. (C) RT-PCR analysis of cDNA from neonatal (P0) diaphragm revealed additional transcription of Cx26, Cx32, Cx37, Cx40 and Cx45 in this tissue (upper gel). PCR with genomic DNA displayed the appropriately sized products of the tested connexins (lower gel).