Fig. 6. Immunolocalization of Cx39 in different developing mouse striated muscles. (A-G) Affinity-purified polyclonal antibodies directed to the C-terminus of mCx39 were used to label 10 µm cryosections of the diaphragm (A-D), the intercostal muscle (E, F) and the hind limb (G). Punctate green staining between myotubes (A,C,E-G) indicated expression of mCx39, which was absent when primary antibodies were omitted (B,D). (H) Immunofluorescence analysis of cultured HeLa cells stably transfected with the coding DNA of mCx39 after incubation with rabbit anti-Cx39 and Alexa488-conjugated goat anti-rabbit immunoglobulin. HeLa mCx39 transfectants showed the expected connexin-specific punctate staining pattern (green) on contacting plasma membranes. Nuclei were counterstained in red with propidium iodide. Bar, 20 µm.