Fig. 7. Immunolocalization of various connexins in embryonic and neonatal diaphragm. (A-C) Expression of Cx26 (A) and Cx32 (B) is restricted to the developing liver and Cx37 to the endothelium (C). (D) Expression of Cx40 was detected in blood vessels. (E) Cx43 localized near the edge of the diaphragm. (F) Expression of the Cx45 gene, indicated by ß-gal staining in tissue from Cx45 (LacZ⁺) mice (Krüger et al., 2000), is confined to the neonatal lung (shown in the upper part above the dotted line) and to smooth muscle cells around blood vessels (Krüger et al., 2000) of the neonatal diaphragm (lower part). Bar, 20 µm.