Fig. 6. Images of 4-day-old GFP clones sequentially labeled with anti-Pax7 followed by anti-MyoD. (A-B') Clone 1 is positive for both Pax7 and MyoD. (C-D') Clone 2 is positive for Pax7 but negative for MyoD. The use of GFP-labeled cells allowed detailed characterization of the cell body, demonstrating morphological distinctions between the two clonal phenotypes. Bar, 20 µm.