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Fig. 5. {alpha}4ß1- or {alpha}6ß1-Integrin precipitates with LRP6. COS-7 cells were co-transfected with LRP6-FLAG and ß1-HA (A,B,G), LRP6-FLAG and {alpha}6-HA (C,D,H) or LRP6-FLAG and {alpha}4-Myc (E,F). Single transfections were also done. After precipitation with anti-FLAG antibody (A,C,E,G,H), anti-HA antibody (B,D) or anti-Myc antibody (F), the immunoprecipitates were probed for the presence of ß1-HA (A,G), {alpha}6-HA (C,H), {alpha}4-myc (E) or LRP6-FLAG (B,D,F) by immunoblotting. In (G), experiments were performed as in (A) except that cells were incubated with 200 ng ml–1 MK for appropriate period. In (H), experiments were performed as in (C) except that the time of transfection was reduced to 1.5 hours and that cells were incubated with 200 ng ml–1 MK for appropriate period.