Fig. 1. Phosphorylation-state-dependent localization of BACE and GGA1. BACE-V5 [wild-type (WT), S498D and S498A] and GGA1-Myc co-transfected N2a cells grown on glass coverslips were immunostained with anti-V5 mAb (visualized using Cy3) and rabbit anti-Myc Ab (visualized using FITC). Notice that the subcellular localization of BACE in juxtanuclear compartments and the partial colocalization with GGA1 is the same in WT, S498D and S498A transfected cells, whereas distribution in the periphery of the cell predominates in the non-phosphorylated BACE mutant (S498A).