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Fig. 6. Immunoprecipitation of BACE phosphorylated at its Serine residue in human brain tissue. (Lane 1) AD human brain. Immunoprecipitation (IP) with ms anti-phosphoserine, probe with rb anti-BACE (N-terminus). (Lane 2) Normal human brain. IP with ms anti-phosphoserine, probe with rb anti-BACE (N-terminus). (Lane 3) Normal human brain. IP with rb anti-BACE (C-terminus), probe with ms anti-phosphoserine. (Lane 4) Normal human brain. IP with ms anti-BACE (C-terminus), probe with rb anti-BACE (N-terminus) (positive control). (Lane 5) BACE standard. Probe with rb anti-BACE (N-terminus) (positive control). (Lane 6) Buffer. IP with ms anti-phosphoserine, probe with rb anti-BACE (N-terminus) (negative control). (Lane 7) Normal human brain. IP with ms anti-phosphotyrosine, probe with rb anti-BACE (N-terminus) (negative control).