Fig. 2. Time course of insulin- and PDGF-induced actin remodeling in myoblasts. Serum-deprived GLUT4myc myoblasts were left untreated (a,f) or stimulated with 100 nM insulin (b-e) or with 50 ng ml^–1 PDGF-BB (g-j) for the times indicated. The cells were then fixed, permeabilized and labeled with rhodamine-conjugated phalloidin. Representative images are shown of a single focal plane near the dorsal surface of the cell. The images shown are representative of five experiments. Scale bar, 10 µm.