JCS -- Raynaud-Messina et al. 117 (23): 5497 Figure IG3

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Fig. 3. Post-metaphase figures observed after 23C- ${gamma}$ -tubulin depletion. Chromosomes appear in blue, microtubules in red, and the staining in green corresponds to ${gamma}$ -tubulin. (A,A') Control cytokinesis showing ${alpha}$ -tubulin and ${gamma}$ -tubulin merged staining (A) and ${gamma}$ -tubulin staining only, showing the presence of 23C ${gamma}$ -tubulin at the two poles and in the mid-body (A'). (B-E) Anaphase (B), telophase (C) and cytokinesis (D,E) after ${gamma}$ -tubulin depletion. None of these figures exhibited ${gamma}$ -tubulin signals (absence of green staining). Cytokinesis was generally symmetrical (D) and exceptionally asymmetrical (E). Scale bar, 5 µm.