Fig. 1. Cells expressing both paxillin-CFP and 5-YFP show visibly organized paxillin but not 5-integrin. (A-D) Expressed paxillin-CFP in a protrusion at various time points of a confocal image time series of MEF cells during adhesion formation and turnover. The pairwise corresponding images of 5-YFP expression for the same regions of the cell are shown in E-H. Cells were 24 hours post-transfection and were plated on 1 µg ml^–1 fibronectin for an hour before imaging at 37°C. Scale bar, 10 µm.