Fig. 6. Disassembly of 5-integrin in a retracting region of the cell. Confocal images of CHO B2 cells stably expressing 5-GFP 3 hours after plating on 2 µg ml^–1 fibronectin. The first (A) and the last image in the 17 minute time series (B) are shown. Plots of the average intensity of the four bright adhesions (filled circles) and of a nonadhesive area (open triangles, region 8) within the boxed region are shown. A single exponential fit was made to the data to determine the rate of adhesion disassembly. (D,E) Outlines of individual adhesions from the boxed region that were analyzed by intensity to provide a measure of integrin density within adhesions (Table 2). Scale bars, 10 µm.