Fig. 6. Cellular localization of endogenous and recombinantly expressed Mint3. Endogenous Mint3 and Bcr were visualized by confocal immunofluorescence microscopy in polarized Calu-3 layers (A) and HEK293 cells (B) as described in Materials and Methods. (C) BHK-21 cells were transiently transfected with Bcr/pcDNA3.1 and Mint3/pCMV5 using LipofectAMINE Plus and proteins were detected 30 hours after transfection.