Fig. 6. Tip1p and Ssm4p collaborate to localise Dhc1p. (A) Localisation of Ssm4p-GFP in the dhc1 and tip1 mutant zygotes. (B) Localisation of GFP-Dhc1p in the ssm4, tip1 and ssm4 tip1 mutant zygotes. (C) cyr1 sxa2 dhc1GFP, cyr1 sxa2 ssm4 dhc1GFP, cyr1 sxa2 tip1 dhc1GFP, cyr1 sxa2 tip1 ssm4 dhc1GFP cells were grown in pheromone for 6 hours and then imaged with a fluorescence microscope. The white arrows indicate the microtubule tips and the yellow arrowheads the SPB. (D) cyr1 sxa2 ssm4 dhc1GFP, cyr1 sxa2 tip1 ssm4 dhc1GFP cells were grown in pheromone for 6 hours and then fixed and stained for tubulin. (E) ssm4 dhc1GFP, tip1 ssm4 dhc1GFP zygotes were fixed and stained for tubulin. (F) Colocalisation of Tip1p and Dhc1p. cyr1 sxa2 tip1YFP dhc1GFP cells were treated with pheromone for 5 hours. Cells were visualised with a confocal microscope using a CFP-YFP filter set which allows to separate the YFP and GFP signals. A single plane through the centre of the cells was taken. Arrows indicate fluorescence at the SPB and arrowheads indicate fluorescence at the microtubular tip. Bars, 10 µm (A,B); 3 µm (in C for C and D; F); 5 µm (E).