(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 8. Microtubules in ssm4{Delta} fission yeast mutants are less dynamic and do not oscillate. (A) cyr1{Delta} sxa2{Delta} ssm4{Delta} nmt1atb2GFP cells were treated with pheromone for 5 hours. Cells were then imaged every 12 seconds on a confocal microscope. Images shown are projections of sections through the whole cell. (B) The lengths of microtubules for single cells were measured distinguishing on which side of the cell they lie. The diagram describes the dynamics of the microtubules of the cell in A. The coordination of dynamics between opposite cell ends was then calculated as a percentage of total time points. Four cells were scored for a total imaging time of 20 minutes. At least 10 cells were observed for a total imaging time of 50 minutes and they all exhibited a similar behaviour. (C) cyr1{Delta} sxa2{Delta} ssm4{Delta} nmt1atb2GFP cells were induced with pheromone for 6 hours. Cells were imaged every 7 seconds on a confocal microscope. Images shown are projections of sections through the cell. The yellow arrow indicates where there is a change of fluorescence along a bundle. (D) cyr1{Delta} sxa2{Delta} ssm4{Delta} nmt1atb2GFP cells were induced with pheromone for 5-6 hours, placed on a lectin-coated glass bottom dish, bleached with a localised laser beam and then filmed every 6 seconds to monitor the pattern of recovery. Images show a single plane through the middle of the cell. The yellow arrows indicate the position of the SPB. The grey lines mark the initial bleached area. The bleached area recovers from the SPB. The time taken by fluorescence to recover over a 2.3 µm bleached area was 43.3±11.1 seconds (n=16) for the wild type (from Fig. 7C) and 40.3±11.3 seconds (n=17) for ssm4{Delta} cells. (E) cyr1{Delta} sxa2{Delta} ssm4{Delta} cells were induced with pheromone for 6 hours. Microtubules were bleached with a pulse of laser light, MBC was then added to depolymerise the microtubules and microtubules were filmed with a confocal microscope. Images shown are single planes through a cell. The yellow arrowheads mark the stud remaining after microtubules had depolymerised. Bar in A,C, 3.5 µm; D,E, 3 µm.