Fig. 9. Microtubular dynamics have lost coordination in ssm4 tip1 cells. (A,B,C) cyr1 sxa2 tip1 nmt1atb2GFP and (D, E) cyr1 sxa2 tip1 ssm4 nmt1atb2GFP cells were treated with pheromone for 5 hours. (A,D) Cells were then imaged every 6 seconds on a confocal microscope. Images shown are projections of sections through the whole cell. Yellow arrows mark the position of the SPB. + and – indicate growing and shrinking microtubules respectively. (B,C,E) Microtubule lengths for single cells were measured distinguishing the side of the cell in which they lie. B describes the dynamics of the microtubules of the cell in A, diagram E corresponds to D, and diagram in C describes a different tip1 cell. The coordination of dynamics between opposite cell ends was then calculated as a percentage of total time points; eight cells were scored for a total imaging time of 45 minutes. Bar, 2 µm.